Identifikasi Bakteri Indigenous Sebagai Agen Bioremediasi di Lahan Pertanian Cabai Merah Keriting (Capsicum Annum) Yang Terpapar Pestisida Profenofos
DOI:
https://doi.org/10.55606/jurrimipa.v5i2.9838Keywords:
Bioremediation, Indigenous Bacteria, Pesticide Degradation, Profenofos, Contaminated SoilAbstract
Indigenous bacteria are microorganisms that naturally live and adapt to specific environments, making them unique to that area. In bioremediation, these bacteria play a crucial role due to their ability to degrade various environmental contaminants, such as the pesticide profenofos left in the soil. This ability makes indigenous bacteria effective and cost-efficient agents in addressing environmental pollution, highlighting their importance in agricultural land recovery research. The aim of this study is to isolate and identify indigenous bacteria from soil exposed to profenofos pesticide and analyze them descriptively. The study is divided into several stages: sample collection, isolation using Nitrate Minerals Salts (NMS) medium, ability testing using Muller Hilton Agar (MHA) medium, characterization, Gram staining, and biochemical testing. The results revealed that 8 isolates have potential as bioremediation agents for soil contaminated with profenofos. In the ability test against the pesticide, the antibacterial inhibition of profenofos was categorized into four levels: weak, moderate, strong, and very strong. The results showed that the isolate with the code BIPC 8 had the highest clear zone diameter of 32.95 mm, indicating a very strong ability. Out of the 8 isolates, 7 were categorized as strong and 1 as very strong, showing potential to reduce profenofos residues in the soil. The identified genera of these isolates include Nitrosoccocus, Bacillus, Pseudomonas, and Rhodococcus. In conclusion, indigenous bacteria are capable of degrading profenofos pesticide.
Downloads
References
Airlangga, S. S. D., Mochammad, M., & Poniman. (2021). Pengaruh pemberian biochar terhadap beberapa sifat biokimia tanah dan pertumbuhan tanaman bawang merah pada lahan tercemar residu pestisida. Jurnal Tanah dan Sumberdaya Lahan, 8(1), 27–34.
Akhdiya, A., Wartono, Eman, S., & Samudra, I. M. (2018). Karakterisasi bakteri pendegradasi profenofos. Jurnal Agrobiogen, 14(1), 37–46.
Amaliah, Z. Z. N., Bahri, S., & Amelia, P. (2018). Isolasi dan karakterisasi bakteri asam laktat dari limbah cair rendaman kacang kedelai. Jurnal Farmasi Fitofarmaka Indonesia, 5(1), 253–257.
Apriliya, I., Dedy, P., & Remila, S. (2020). Isolasi bakteri rizosfer resisten pestisida dan herbisida pada berbagai jenis tutupan lahan. Jurnal Agroteknologi dan Ilmu Pertanian, 5(1), 2548–7841.
Ari, S. (2021). Perlu bijak dalam penggunaan pestisida. Dinas Ketahanan Pangan Pertanian Perikanan Kabupaten Temanggung.
Asri, H. T., Yuliani, Tarsan, P., Fida, R., Evie, R., & Agoes, S. (2019). Potential of Indonesian endemic microbial consortium in degrading profenofos and chlorantraniliprole pesticide in East Java Indonesia to support agricultural ecosystems. Eco. Env. & Cons, 25, S85–S90.
Ayilara, M., & Olubukola, O. B. (2023). Bioremediation of environmental wastes: The role of microorganisms. Frontiers in Agronomy, 5, 1183691.
Bansal, O. P. (2011). Fate of pesticides in the environment. Journal of the Indian Chemical Society, 88(11), 1525–1532.
Benu, M. M. M., Anthonius, S. J. A. T., & Lince, M. (2019). Dampak residu insektisida terhadap keanekaragaman jamur tanah pada lahan sayuran. Jurnal Bumi Lestari, 19(2), 20–30.
Benu, M. M. M., Anthonius, S. J. A. T., & Lince, M. (2020). Dampak residu insektisida terhadap keanekaragaman jamur tanah pada lahan sayuran sawi. Jurnal Ilmu Tanah dan Lingkungan, 22(2), 80–88.
Brooke, J. S. (2021). Advances in the microbiology of Stenotrophomonas maltophilia. Clinical Microbiology Reviews, 34(19).
Cho, S. J., Kim, M. H., & Lee, Y. O. (2016). Effect of pH on soil bacterial diversity. Journal of Ecology and Environment, 40, 10.
Damayanti, S. S., Komala, O., & Effendi, E. M. (2018). Identification bacteri of liquid organic fertilizer the contents of cow rumen. Jurnal Ilmiah Ilmu Dasar dan Lingkungan Hidup, 18.
Darma, A., Jianjung, Y., Peiman, Z., Jin, L., Katarzuna, M., Xing, X., Ali, S., Yihao, W., & Ewald, S. (2022). Significance of Shewanella species for the phytoavailability and toxicity of arsenic—A review. PMC, 11(3), 472.
Davis, W., & Stout, T. (1971). Disc plate method of microbiological antibiotic assay. Applied Microbiology, 22(4), 659–665.
Departemen Agama RI. (2009). Al-Qur’an dan tafsirnya. Lembaga Percetakan Al-Qur’an Departemen Agama.
Devianti, D., Sufardi, Zulfahrizal, & Agus, A. Munawar. (2019). Near infrared reflectance spectroscopy: Prediksi cepat dan simultan kadar unsur hara makro pada tanah pertanian. Agritech, 39(1), 12–19.
Donley, N., Robert, D. B., Jeannie, E., Iris, F., Jovita, L., Amy K. L., Dominica, N. M., & Fatemeh, S. (2022). Pesticides and environmental injustice in the USA: Root causes, current regulatory reinforcement and a path forward. BMC Public Health, 22, 708.
Efendi, Y. O., & Yempita, E. (2013). Mikrobiologi hasil perikanan (Jilid I). Bung Hatta University Press.
Fishel, F. M. (2020). Pesticide characteristics. University of Florida IFAS Extension.
Handayani, Rita, S., Marli, M., Nurlita, P., Riyanti, Lukman, B. A., Tarzan, P., & Ilham, R. (2022). Teknik lingkungan. Global Eksekutif Teknologi.
Hidayati, D. B. I. (2019). Intoksikasi organofosfat dengan krisis kolinergik akut, gejala peralihan dan polineuropati tertunda. Jurnal Agromedicine, 6(2), 334–342.
Indratin, Asep, K., & Sri, W. (2019). Degradation of cypermethrin by indigenous bacteria from contaminated soil. Makara Journal of Science, 23(4), 210–216.
Jawetz, E., Melnick, J. L., & Adelberg, E. A. (2005). Mikrobiologi kedokteran. Salemba Medika.
Karanasios, E., Dimitrios, G. K., & Nikolaos, G. T. (2012). Key parameters and practices controlling pesticide degradation efficiency of biobed substrates. Journal of Environmental Science and Health, Part B, 47(6), 589–598.
Keman, S. (2022). Dasar kesehatan lingkungan. Airlangga University Press.
Kim, D., Ki, Y. C., Miyoun, Y., Gerben, J. Z., & Eungbin, K. (2018). Biotechnological potential of Rhodococcus biodegradative pathways. Journal of Microbiology and Biotechnology, 28(7), 1037–1051.
Kosasi, C., Lolo, W. A., & Sudewi, S. (2019). Isolasi dan uji aktivitas antibakteri dari bakteri yang berasosiasi dengan alga Turbinaria ornata (Turner) J. Agardh serta identifikasi secara biokimia. Pharmacon, 8(2), 351.
Mpapa, B. (2016). Analisis kesuburan tanah tempat tumbuh pohon jati (Tectona grandis L.) pada ketinggian yang berbeda. Jurnal Agrista Unsyiah, 20(3), 135–139.
Novia, W., & Fajriani. (2021). Analisa perbandingan kadar keasaman (pH) tanah sawah menggunakan metode kolorimeter dan elektrometer di Desa Matang Setui. Jurnal Hadron, 3(1), 10–12.
Nursyam, H., & Prihanto, A. (2018). Identifikasi molekuler bakteri endofit mangrove Rhizophora mucronata penghasil gelatinase (MMP2). Jurnal Pengolahan Hasil Perikanan Indonesia, 21(1), 143–147.
Oktavia, R., Endang, N., Sri, W., & Sumardi. (2022). Kemampuan Bacillus sp. sebagai bioremediasi bahan pencemar. Jurnal Bioterdidik, 10(2), 110–124.
Onwuka, B., & Brown, M. (2018). Effects of soil temperature on some soil properties and plant growth. Advances in Plants & Agriculture Research, 8(1), 34–37.
Ore, O. T., Adedapo, O. A., Ajibola, A. B., Demilade, T. A., & Philiswa, N. N. (2023). Organophosphate pesticide residues in environmental and biological matrices: Occurrence, distribution and potential remedial approaches. Environmental Chemistry and Ecotoxicology, 5, 9–23.
Paker, P. N., Shehzad, M., Muhammad, T. J., Christis, A. D., Fazal, U. R., Hassan, J. C., Muhammad, Z. M., & Mahrukh, M. (2023). Elucidating molecular characterization of chlorpyrifos and profenofos degrading distinct bacterial strains for enhancing seed germination potential of Gossypium arboreum L. Environmental Science and Pollution Research.
Patil, Y., & Prakash, R. (2013). Applied bioremediation: Active and passive approaches. IntechOpen.
Patmawati, Y. (2022). Bioremediasi anaerob aerob limbah cair tahu. CV Literasi Nusantara Abadi.
Paul, E. A. (2007). Soil microbiology, ecology and biochemistry (3rd ed.). ScienceDirect.
Pera, A. (2018). Pencemaran pada tanah. Dinas Lingkungan Hidup Kabupaten Badung.
Peraturan Menteri Pertanian Nomor 07/Permentan/SR.140/2/2007 tentang syarat dan tata cara pendaftaran pestisida. (2007). Kementan RI.
Pratiwi, W., & Mahanani, T. A. (2022). Isolasi dan identifikasi bakteri indigenous pendegradasi pestisida profenofos dan klorantraniliprol di Jombang Jawa Timur. Lentera Bio, 11(2), 300–309.
Prihanto, A., & Pradarameswari. (2018). Isolasi dan identifikasi bakteri endofit mangrove Sonneratia alba penghasil enzim gelatinase dari Pantai Sendang Biru Malang. Indonesian Journal of Halal, 2(2).
Puspitasari, D. J., & Khaeruddin. (2016). Kajian bioremediasi pada tanah tercemar pestisida. Kovalen, 2(3), 98–106.
Rahmadian, C. A., Ismail, Mahdi, A., Erina, Rastina, & Yudha, F. (2018). Isolasi dan identifikasi bakteri Pseudomonas sp. pada ikan asin di Tempat Pelelangan Ikan Labuhanhaji Aceh Selatan. Jurnal Ilmiah Mahasiswa Veteriner, 2(4), 493–502.
Rahmiyah, M., Ulfa, W., Asri, A., Dewi, S., Eka, Y., Tili, K., Ika, P., Andini, H., Evan, P. R., Sri, R. F. P., Cheppy, W., Rudi, H., & Dewi, M. (2021). Pengendalian hama dan penyakit tanaman. Yayasan Kita Menulis.
Ratnaningsih, H. R., Prameswari, D. A., & Taopan, R. A. (2020). Isolasi bakteri pendegradasi pestisida dan herbisida. Science Tech: Jurnal Ilmu Pengetahuan dan Teknologi, 6(1), 17–25.
Rebello, S., Vinod, K. N., Raveendran, S., Parameswaran, B., Mukesh, K. A., & Ashok, P. (2021). Bioengineered microbes for soil health restoration: Present status and future. Bioengineered, 12(2), 12839–12853.
Rita, H. (2022). Analisis produksi cabai Provinsi Sumatera Utara 2021. Badan Pusat Statistika Provinsi Sumatera Utara.
Rohde, M. (2019). The Gram-positive bacterial cell wall. Microbiology Spectrum, 7(3), 10–11.
Rukmana, A., Helfy, R., & Ginting. (2019). Pencatat pH tanah otomatis. Jurnal Penelitian dan Pengembangan Teknik Elektro Telekomunikasi Indonesia, 10(1), 25–33.
Saraswati, R., Edi, H., & Simanungkalit, R. D. M. (2007). Metode analisa biologi tanah. Balai Besar Penelitian dan Pengembangan Sumberdaya Lahan Pertanian.
Setiyo, Y., Gunam, I. B. W., Gunadnya, I. B. P., & Tika, I. W. (2016). Bioremediasi in situ lahan tercemar pestisida oleh mikroba yang ada pada kompos. The Excellence Research Universitas Udayana.
Sharma, A., Vinod, K., Babar, S., Mohsin, T., Gagan, P. S. S., Neha, H., Sukhmeen, K. K., Poonam, Y., Aditi, S. B., Ripu, D. P., Owias, I. D., Kirpal, S., Shivam, J., Palak, B., M. R., Sandeep, K., Renu, B., & Ashwani, K. T. (2019). Worldwide pesticide usage and its impacts on ecosystem. A Springer Nature Journal, 1, 1446.
Sharma, P., & Kumar, S. (2023). Soil temperature and plant growth. In Soil physical environment and plant growth. Springer.
Shen, X., Junwei, M., Yuqian, L., Yijia, L., & Xinghui, X. (2022). The effects of multiple global change factors on soil nutrients across China: A meta-analysis. International Journal of Environmental Research and Public Health, 19(22), 15230.
Sianipar, G. W. S., Sartini, & Riyanto. (2020). Isolasi dan karakteristik bakteri endofit pada akar pepaya (Carica papaya L.). Jurnal Ilmiah Biologi UMA, 2(2), 83–92.
Siswanto, B. (2019). Sebaran unsur hara N, P, K dan pH dalam tanah. Jurnal Buana Sains, 18(2), 109.
Souisa, G. V., Bellytra, T., & Zasendy, R. (2020). Peningkatan perilaku pencegahan dampak pestisida pada kesehatan petani. Jurnal Pengabdian kepada Masyarakat, 26(3), 109–113.
Sri, J. (2022). Dampak residu pestisida pada pangan segar asal tumbuhan. Dinas Ketahanan Pangan Kabupaten Banyuasin.
Supriatna, Sondang, S., & Indah, R. (2021). Pencemaran tanah oleh pestisida di perkebunan sayur Kelurahan Eka Jaya Kecamatan Jambi Selatan Kota Jambi. Jurnal Ilmiah Universitas Batanghari Jambi, 21(1), 460–466.
Tudi, M., Huada, D. R., Li, W., Jia, L., Ross, S., Des, C., Cordia, C., & Dung, T. P. (2021). Agriculture development, pesticide application and its impact on the environment. International Journal of Environmental Research and Public Health, 18(3), 1112.
Velez, J. M. B., Jose, G. M., Juliana, T. O., & Susana, O. A. (2021). Bioremediation potential of Pseudomonas genus isolates from residual water, capable of tolerating lead through mechanisms of exopolysaccharide production and biosorption. Biotechnology Reports, 32, e00685.
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2026 Sarah Safira, Kartika Manalu, Rizki Amelia Nasution
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
