Penetapan Kadar Fenolik Total Ekstrak Daun Jotang (Sphagneticola Trilobata) Dengan Spektrofotometri UV-Vis
DOI:
https://doi.org/10.55606/jurrikes.v2i1.2669Keywords:
Jotang, Sphagneticola trilobata, Total phenolics, Folin ciocalteauAbstract
Phenolic compounds are the largest group of compounds that act as natural antioxidants in plants. One of the plants that has the potential as a natural antioxidant is Jotang (Sphagneticola trilobata). This study aims to determine whether jotang leaf extract (Sphagneticola trilobata) has phenolic content and to determine the total phenolic content in jotang leaf extract (Sphagneticola trilobata). Jotang leaves were extracted by maceration method using methanol solvent. The method of determining total phenolic content was carried out using Folin-ciocaltaeu reagent because phenolic compounds can react with Folin-Ciocalteu to form a solution that can be measured absorbance. As a standard solution, gallic acid is used which is reacted with Folin-Ciocalteau reagent to produce a yellow color indicating that it contains phenolics, after which it is added with Na₂CO₃ solution as a base atmosphere giver. The phytochemical screening of jotang leaf extract showed positive results containing flavonoids, alkaloids, saponins, phenolic compounds, and triterpenoids. Determination of the wavelength of gallic acid obtained maximum 653 nm. The results showed that jotang leaf extract (Sphagneticola trilobata) has phenolic content and obtained total phenolic content of jotang leaf extract of 0.909 mg /g.
References
Ahmad, A.R., Juwita., Ratulangi, D., S. A., Malik, &, & A. (2015). Penetapan Kadar Fenolik dan Flavonoid Total Ekstrak Buah dan Daun Patikala (Etlingera elatior (Jack) R.M.SM). Pharmaceutical Sciences and Research, 2(1), 1-10.
Aliah, N., Susilawaty, A., & & Ibrahim, I. (2016). Uji Efektivitas Ekstrak Daun Cengkeh (Syzigium aromaticum) Sebagai Repellent Semprot Terhadap Lalat Rumah (Musca Domestica). 2(3), 113–120.
Aryal, S., Baniya, M. K., Danekhu, K., Kunwar, P., Gurung, R., & dan Koirala, N. (2019). Total phenolic content, flavonoid content and antioxidant potential of wild vegetables from western nepal. Plants, 8(96), 1–12.
Balekar, N, Nadpi Gangadhar Katkam, Titpawan Nakpheng, Kholeeyoh Jehtae, Teerapol Srichana. Evaluation Of The Wound Healing Potential Of Wedelia trilobata (L) Leaves. Journal Of Ethnopharmacology 141 (2012) 817-824.
Chowdhury, M. F. M., & Lavelli, A. (2013). Fbk-Irst: A Multi-Phase Kernel Based Approach For Drug-Drug Interaction Detection And Classification That Exploits Linguistic Information. Second Joint Conference On Lexical And Computational Semantics ,. Proceedings Of The Seventh International Workshop On Semantic Evaluation, 2, 351–355.
Chun, O.K., Kim, D.O., Lee, A., & C.Y. (2003). Superoxide Radical Scavenging Activity of The Major.
Dachriyanus. (2004). Analisis Struktur Senyawa Organik Secara Spektroskopi. Departemen Kesehatan RI. 1995. Farmakope Indonesia Edisi IV. Derektorat Jendral Pengawasan Obat dan Makanan : Jakarta. Hal: 7, 1221-1223.
Departement Kesehatan RI. (2000). Parameter Standart Umum Ekstrak Tumbuhan Obat, Cetakan Pertama, 3-11, 17-19. Ditjen POM. Direktorat Pengawasan Obat Tradisional.
Endarini, & Hanni, L. (2016). Modul Bahan Ajar Cetak Farmasi. Farmakognosi Dan Fitokimia. Jakarta Selatan : Pusdik SDM Kesehatan.
Gandjar, & Gholib., I. (2007). Kimia Farmasi Analisis. Yogyakarta : Pustaka Pelajar.
Gauglitz, G., & Vo-Dinh., and T. (2003). Handbook of Spectroscopy. Wiley-VCH. Weinheim, Jerman. Pp. 89;125;129; and 347.
Hanani. (2015). Analisis Fitokimia. Buku kedokteran EGC: Jakarta.
Hagerman, A. E. (2002). Tanin Chemistry Handbook. Department of Chemistry and Biochemistry, Miami University.
Harborne, J. (2006). Metode Fitokimia: Penuntun Cara Modern Menganalisis Tumbuhan (alih bahasa: Kosasih Padmawinata & Iwang Soediro). Penerbit ITB.
Harborne, J. B. (1987). Metode Fitokimia. Penerjemah Padmawinata I.K. Soediro Institut Teknologi Bandung, Bandung.
Huang. (2005). Identification of an Antifungal Chitinase from a Potential Biocontrol Agent, Bacillus cereus.
Khumaira Sari, A., Aisyah, N., & Prihandiwati Akademi Farmasi ISFI Banjarmasin, E. (2019). Penentuan Kadar Fenolik Total Ekstrak Etanol 96% Daun Terap (Artocarpus Odoratissimus Blanco) Dengan Metode Spektrofotometri Visible. Jurnal Ilmiah Ibnu Sina, 5(1). https://doi.org/10.36387/jiis.v5i1.417
Kristanti, A. N., Aminah, N. S., Tanjung, M., & Kurniadi, B. (2008). Buku Ajar Fitokimia. Surabaya, Airlangga University Press, hal 23.
Lumbantobing, H. (2010). Analisa Komposisi Minyak Atsiri Bunga Jotang (Spilanthes paniculata) Dengan Menggunakan Spektometer GC- MS. Thesis. Fakultas MIPA Universitas Sumatera Utara.
Nilapwar, S. M., Nardelli, M., Westerhoff, H. V., &, & Verma, M. (2011). Absorption Spectroscopy. Methods in Enzymology, 500, 59-70.
Ramadhani, A., Saadah, S., & & Sogandi, S. (2020). Efek Antibakteri Ekstrak Daun Cengkeh (Syzygium Aromaticum) Terhadap Escherichia coli Dan Staphylococcus aureus. Jurnal Bioteknologi & Biosains Indonesia (Jbbi), 7(2), 203–214.
Redha, A. (2010). Flavonoid: Struktur, Sifat Antioksidatif dan Peranannya Dalam Sistem Biologis. Jurnal Berlin, 9(2), 196–202.
Robinson, T. (1995). Kandungan organik tumbuhan tinggi. Institut Teknologi Bandung .
Silalahi, J. (2006). Makanan Fungsional. Kanisius.Yogyakarta.
Soltani, M., Parivar, K., Baharara, J., Kerachian, A. A., & & Asili, J. (2014).
Hemolytic And CytotoxicProperties Of Saponin Purified From Holothuria‘LeucospilotaSea Cucumber. Reports of Biochemistry and Molecular Biology, 3(1:), 1–8.
Suhartati, T. (2017). Dasar-Dasar Spektrofotometer UV-VIS dan Spektrofotometri Banjarmasin, E. (2019). Penentuan Kadar Fenolik Total Ekstrak Etanol 96% Daun Terap (Artocarpus Odoratissimus Blanco) Dengan Metode Spektrofotometri Visible. Jurnal Ilmiah Ibnu Sina, 5(1). https://doi.org/10.36387/jiis.v5i1.417 Massa Untuk Penentuan Struktur Senyawa Organik. Lampung: AURA.
Suharti, T. (2013). Dasar-Dasar Spektrofotometri UV-Vis dan Spektrometri Massa untuk Penentuan Struktur Senyawa Organik. Bandar Lampung : CV. Anugrah Utama Raharja.
Tahir, M., Mulflihunna, A., & Syafrianti, S. (2017). Penenrtuan Kadar Fenolik Total Ekstrak Etanol Daun Nilam (Pogostemon cablin Benth.) Dengan Metode Spektrofotometri UV-Vis. Jurnal Fitofarmaka Indonesia , 4(1), 215-244. https://doi.org/10.33096/jffi.v4il.231.
Thomas, T. (2011). Antibacterial action of gradient extracts of flower heads of Spilanthes paniculata Wall. Ex DC. Plant Sciences Feed, 1(11), 186–189.
Voigt, T. 1994. Buku Pelajaran Teknologi Farmasi Edisi V. Ahli Bahasa Noerono, S. Universitas Gajah Mada Press : Yogyakarta. Hal. 564.
Wirajana, I. N. (n.d.). Jurnal Kimia. ( Journal of Chemistry, 8. ).
Saifudin, A., Rahayu, & Teruna. 2011. Standarisasi Bahan Obat Alam. Graha Ilmu : Yogyakarta.
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2023 Ghalib Syukrillah Syahputra, Delladari Mayefis, Tommy Julianto, Lyna Elvira
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
