Analisa In Silico Senyawa Biji Lada Hitam (Piper nigrum L.) Terhadap Aktivitas Antioksidan
DOI:
https://doi.org/10.55606/jurrikes.v2i2.1815Keywords:
Antioxidant, ascorbic acid, in silico, black pepper, molecular dynamicsAbstract
Degenerative disease is a health condition of an organ or related tissue whose condition can deteriorate over time due to changes in body cells that affect organ function as a whole. The factor is due to unhealthy life changes such as lack of activity and frequent exposure to harmful substances into the body. Degenerative diseases include hypertension, diabetes, cholesterol, cancer, and death. Harmful substances or free radicals can be inhibited by antioxidants. One drug that has antioxidant activity is ascorbic acid (Vitamin C) which functions as an immune booster and is used by the public because it is effective as an antioxidant and health therapy but there are side effects in the form of gastrointestinal. The research aimed to find candidate drug ingredients from compounds contained in black pepper seeds (Piper nigrum L.) as in silico antioxidants. The methods used are molecular docking and molecular dynamics. The results showed BLH19 had better toxicity than the comparator drug and had hydrogen interactions that could be stabilized by the amino acid Gln139. The results of molecular dynamic calculations for BLH19 have an RMSD value of 1.5 Å with a ΔGTotal of -33.50 Kcal/mol. While the comparator drug has an RMSD value of 1.6 Å with ΔGTotal -3.39 Kcal/mol. The results showed that the affinity level of ascorbic acid compared to BLH19 for the 4RP9 receptor was predicted to be a candidate for a new antioxidant drug.
References
Aghnia Salsabila, H., Author, C., Studi Pendidikan Dokter, P., Kedokteran, F., & Lampung, U. (2021). Efektivitas Ekstrak Lada Hitam (Piper Nigrum L.) Dan Zink (Zn) Terhadap Viabilitas Dan Morfologi Sperma. Jurnal Medika Hutama, 3(01): 1507-1511.
Alaris D, D., Ricky J., Fitriyani., Adang., Wiwin W. (2020). Peningkatan Kelarutan Ekstrak Lada (Piper Nigrum L.) Dalam Air Dan Karakterisasinya. Jurnal Sains dan Teknologi Farmasi Indonesia, 9(1) : 61-74.
Budilaksono, W., Wahdaningsih, S., Fahrurroji, A., & Studi Farmasi, P. (2014). Uji Aktivitas Antioksidan Fraksi N-Heksanakulit Buah Naga Merah (Hylocereus Lemairei Britton Dan Rose) Menggunakan Metode DPPH (1,1-Difenil-2- Pikrilhidrazil). Jurnal Mahasiswa Farmasi Fakultas Kedokteran UNTAN, 1(1): 1-11.
Dermawan, D., Sumirtanurdin, R., & Dewantisari, D. (2019). Molecular Dynamics Simulation Estrogen Receptor Alpha againts Andrographolide as Anti Breast Cancer. Indonesian Journal of Pharmaceutical Science and Technology, 6(2): 65-76.
Ferreira, L. G., dos Santos, R. N., Oliva, G., & Andricopulo, A. D. (2015). Molecular docking and structure-based drug design strategies. In Molecules, 20(7) : 13384– 13421).
Guan, L., Yang, H., Cai, Y., Sun, L., Di, P., Li, W., Liu, G., & Tang, Y. (2019). ADMET- score-a comprehensive scoring function for evaluation of chemical drug-likeness. MedChemComm, 10(1) : 148–157.
Irwan I . (2021). Penelitian dan Pengembangan Kefarmasian, L., & Tropis, F. Journal homepage., 92–98.
Juliana, K., Amin, M., & Suarsini, E. (2016). Analisis Virtual Screening Senyawa Alami Anti Aging Kandidat Inhibitor Komplek Protein Mmp1. Seminar Nasional Pendidikan dan Saintek, 114-125
Karsiwan., Lisa Retno., & Tusriyanto. (2022). Jejak Perkembangan Lada Masa Kesultanan Banten di Lampung th 1662-1772. Candrasangkala: Jurnal Pendidikan dan Sejarah, 8 (2):50-62
Kesuma, D., Siswandono, S., Purwanto, B. T., & Hardjono, S. (2018). Uji in silico Aktivitas Sitotoksik dan Toksisitas Senyawa Turunan N-(Benzoil)-N’- feniltiourea Sebagai Calon Obat Antikanker. JPSCR : Journal of Pharmaceutical Science and Clinical Research, 3(1): 1-11.
Kurniasih, N., Kusmiyati, M., Nurhasanah., Puspita Sari, R., Wafdan, R. (2015). Potensi Daun Sirsak (Annona Muricata Linn), Daun Binahong (Anredera Cordifolia (Ten) Steenis), Dan Daun Benalu Mangga (Dendrophthoe Pentandra) Sebagai Antioksidan Pencegah Kanker. Jurnal Istek, 9(1) : 162-184.
Labagu, R., Asri, Naiu, S., Yusuf, N., Teknologi, J., Perikanan, H., Perikanan, F., & Kelautan, I. (2022). Kadar Saponin Ekstrak Buah Mangrove (Sonneratia Alba) Dan Daya Hambatnya Terhadap Radikal Bebas DPPH Levels Of Saponin In Mangrove Fruit (Sonneratia Alba) Extract And Its Inhibition Against Dpph Free Radical. Jambura Fish Processing Journal, 4(1): 1–11.
Luo, P., Yu, X., Wang, W., Fan, S., Li, X., & Wang, J. (2015). Crystal structure of a phosphorylation-coupled vitamin C transporter. Nature Structural and Molecular Biology, 22(3), 238–241.
Mardianingrum, R., Bachtiar, K. R., Susanti, S., Aas Nuraisah, A. N., & Ruswanto, R. (2021). Studi In Silico Senyawa 1,4-Naphthalenedione-2-Ethyl-3-Hydroxy sebagai Antiinflamasi dan Antikanker Payudara. ALCHEMY Jurnal Penelitian Kimia, 17(1), 83–95.
Mighra, B. A., & Djaali, W. (2019). Peningkatan Pengetahuan Lansia tentang Penyakit Degeneratif di Wilayah Kampung Tengah KramatJati. Jurnal Pemberdayaan Komunitas MH Thamrin, 1(2): 49-55.
Muttaqin, F. Z., Ferdian Pratama, M., Kurniawan, F., Tinggi, S., & Bandunng, F. (2019). Molecular Docking And Molecular Dynamic Studies Of Stilbene Derivative Compounds As Sirtuin-3 (Sirt3) Histone Deacetylase Inhibitor On Melanoma Skin Cancer And Their Toxicities Prediction In Molecular Docking. Journal of Pharmacopolium 2 (2) :119-121.
Pangastuti, A., & Amin, M. (2016). Prosiding Seminar Nasional II Tahun (Vol. 26).
Pendidikan Biologi, P., & Wijaya Kusuma Surabaya, U. (2018). Seminar Nasional VI Pengembangan tehnik Docking Molekuler untuk meningkatkan pemahaman kerja enzim dalam pembelajaran biokimia. Seminar nasional VI Prodi Pendidikan Biologi Universitas Muhammadiyah Malang, 8(1): 201-206.
Rahmadi, A., & Yusuf, B. (2018). Book Pangan Fungsional Berkhasiat Antioksidan. Universitas Mulawarman Samarinda. : 1-216.
Ruswanto, R., Mardhiah, M., Mardianingrum, R., & Novitriani, K. (2015). Sintesis dan Studi In Silico Senyawa 3-Nitro-N’-[(Pyridin-4-Yl) Carbonyl]Benzohydrazide Sebagai Kandidat Antituberkulosis. Chimica et Natura Acta, 3(2): 54-61.
Qurrota A’yuna, A., Annisaa’, E., & Dianingati, R. S. (2022). Hubungan Tingkat Pengetahuan Terhadap Perilaku Penggunaan Suplemen Vitamin C Pada Masyarakat Saat Pandemi Covid-19: Di Daerah Kecamatan Lamongan Kabupaten Lamongan. Generics : Journal of Research in Pharmacy, 2(2 : 93-98.
Sartika, D. M., Malaha, N., Sima, Y., & Amanah Makassar Abstrak, S. (2023). Upaya Penguatan Kualitas Kesehatan Dalam Pencegahan Penyakit Degeneratif. Sahabat Sosial Jurnal Pengabdian Masyarakat, 1(2): 1-6.
Siagian, D. D., Kepel, B. J., Manampiring, A., Bodhi, W., & Budiarso, F. D. (2022). Analisis Antioksidan Senyawa Bioaktif Ekstrak Lidah Buaya Secara In silico. E Biomedik, 10(2): 129–135.
Wibawa, J. C., Wati, L. H., & Arifin, M. Z. (2020). Mekanisme Vitamin C Menurunkan Stres Oksidatif Setelah Aktivitas Fisik. JOSSAE : Journal of Sport Science and Education, 5(1): 57-63.
Zulfa, A., Anggun, L., & Murhayati hasriyani, R. (2020). Uji Aktivitas Antibakteri Ekstrak Etanol 70% Biji Lada Hitam (Piper Nigrum L) Terhadap Bakteri Escherichia Coli. In Indonesia Jurnal Farmasi, 5(2): 14-18.
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2023 Elis Nurul Ikhlas, Lina Rahmawati Rizkuloh, Richa Mardianingrum
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
